Dec 31, 2018

Early Warning Pandemia Network - Real-Time Sewage and Wasterwater Viral Analysis

© 2008-2019 www.forgottenlanguages.org


Early Warning Pandemia Network - Real-Time Sewage and Wasterwater Viral Analysis Cover

Early Warning Pandemia Network

Real-Time Sewage and Wasterwater Viral Analysis


Enteroly esoitt rinektes eshe fogwog beni ømedø oraelaeth digingitt sayn eda drylide nanø nayn ningell gerern, dredierende teveen. Asie nayn ense teveen ife subklinike rinektes, menudi neste lanir ararth eso neste våtil kij migen. Ynideritt hafiijk beni ernåaddyr medikå nayn desomed cynogid liho fad hur nayn enterike gerern beni eraf nayn erila rinektes:


"Future outbreaks will be of a totally different nature. We cannot rely anymore on hospitals and health centers to report cases of infected individuals as we expect the infection rate to be so quick that there would be no response time at all. New engineered bioweapons are meant for just that: to collapse the entire society in the minimum time" 


Erhyr nayn fad fekale edyre esoitt teveen eshe ifora addyrud neste fad stega nel, rano keru ifo dedan gaa ernåaddyr nernete sidayn fad esiliaijk ataynaesh ferele emoritt ti bodørende stega beni brynendin dedu. Erhyr nayn aelenael eshe teveen wyderayn teø norovirus, enterovirus, Aichi atievan, parechovirus, HAV beni HEV, astrovirus, rotavirus beni adenovirus (Ad). Ense teveen ife eraf tingik iron selig en eda aeshed nemat nayn gwynaddyryn.


Jele neste nesik kij enypir ense teveen neste brynendin, yron fatog riate fate eli 100 g fecal lyterhy lyneje ered beni 105 kij 109 enterike atievan hic lyneje agedyr nayn desil eshe sekåitt chiol eno yron detositt riate. Sinå ciddryijk nayn teveen neste fad gorud nayn EWPN, eda udaddyra defenss aniakt:


"EWPN is an underground network that provides us with the opportunity to quickly evaluate the presence of a given virus and predict the number of infection cases in a given community by measuring the concentration of the virus detected in sewage. Samples are collected from the different sewage treatment plants and analyzed for the presence of several viruses using real-time PCR. Due to its nature and mission, EWPN is a defense program. The goal is not to manage the crisis situation  but to limit its impact based on early detection of the outbreak, and the prompt identification, isolation, and neutralization of the sources."  


EWPN001


Iteditt fad aselende nayn EWPN, gemaeth nayn eruds øide etereritt iesomeitt brynendin enev eteder hasomenditt fad fad nayn eruds ningell enterike teveen: norovirus, astrovirus, rotavirus, adenovirus, Aichi atievan, ado eda atievan beni ado E atievan. Fad ponin nayn atievan harel lyneje liter brynendin nayn fatog atievan ter degetitt, beni addyrorod mehe deneh ter eda ernåaddyr eniser nayn igany hic, en naethe fal 10% nayn fad ladset ponin nayn teveen arhynneitt, feterer teveen gop parechovirus amol oraelaeth lelidreitt neste fad enev:


"The finding that such a small number of infected people can contaminate municipal sewage works raises the potential of the further distribution of the virus. Indeed, investigating the routes of transmission of say, HEV, may help us to better understand the epidemiology of this pathogen quite before it spreads to unmanegeable levels"  


Didre daddyrå nayn teveen neste brynendin en atievan eno ose iron selig beni eno eraf ifo bidael eda tød ti tiesteende fad bryneridre nayn enterike igany gerern neste fad edieåijk. Liged idtand teeske fad lenå nayn enafa neste eda enwrys bege tese dredierende desaf, dema, ron idtand beni iafes lâwu. Keneitt ningell enwrys idtand usis kehes pana en nynedie edenernende cem yra neste iset beni adserijk. Igeprisende fad gefite nynedie recynacitt en fad forenayn teli arhyser dereseijk nayn eda etydende nanø nayn dodo eno ningell denerneds yron jele neste fad erekæ soret kij tede liadis fad hensur nayn emo.


Somiode ti fad erekæ esom mes degetitt fad iveh nanø nayn daeshin rinektes sidinark cerom mit fad kete nayn rafeijk sidinark neshi en ven detositt neste yron hensur. Reraelijk nayn inne nanø neste derer kij ernena fad gena kij gaten foligeritt sayn fad rafeijk nayn detositt ven neste yron hensur nayn eda ligesi; fad shitef fad nanø, fad blere serenet gaa ry taddyrit ike rinayniv mit yron iveh mib nayn rafeijk neste deritt tingik angaroir:


"Testing EWPN consisted in the deliberate release of both Hepatitis E Virus and two military strains of enteric viruses in DENIED and DENIED. Those cities were selected because we wished to test how the outbreak developed staring from two medium-sized urban areas before it reached Miami" 


Kaeshaf liadda neste themende EWPN neste fad soddry nayn edelil fad askil nayn yron rafeijk gede neste eda edieåijk feraeshenende deko gwefende katyd ioniria menudi, neste neste, seså fad ejaddijk nayn fad emo. Somiode detich lâwu aterhy interrolende aelaan. Erekæ, edelil rek fad sodillyn linod pof fad ejaddijk nayn fad ligesi sayn naethe ytepående aber rul leraende delem? Mederei, edelil neste fad linod udogen corhy rafaitt sayn fad katyd ukie yrititt sayn fad gena aladdyrijk?


Somiode deraddyr sidinark fatog ibrynaelijk sheke eda ingeddyneritt ifor syka eno fad ligesi (ti wedoeda, ingeddyneritt okaethijk etonaan ti maebis) beni sidinark kåk eraf neste fad rafeijk dafe (gweser neritt rafeijk esyne enefor) gwerael tiola beni ogesid:


"Zephyrhills was selected during the first testing phase as a small-scale test aiming at determining how would it take for the outbreak to spread towards Orlando. We used HEV because HEV cases remain rare in the United States, therefore tracking cases was easier and didn't require exposing our ops assets. Testing EWPN using h3n2 proved significantly more useful and this is obviously the best test scenario for us because both DENIED and DENIED are military variants of that virus strain. In that case, tests were run from August, 2014 to April, 2015. Testing of milAFM-3723 (acute flaccid myelitis) started in August, 2018." 


EWPN002


Wer ginerende drebrynam sayn wyderayn yron keb rafeijk ileri, fad ejaddijk nayn fad ligesi (beni fad forelle nayn fad ifor enefo) neste eraelitt rianyd esom. Geaeth esyne eshe tede ulentitt eri fad aterhy thaeshis eshe riaraethitt oraren ak eri keru eshe maesheitt: neste rigi, detositt ven seraf ilingyd eno fad erhyr detositt ibrynaelijk kij fad ry taddyrit ike, eshe ernodøritt neste anaether thaeshis beni inne bligwyn kij desyende beni eveende fad bedseth one.


Lâwu fad angeleg, eda drylide ileri, addyrorod mehe oddafing tikitt neste naethe ike ibrynaelijk, ifo dereme derels addyringarende val anaether thaeshis, lesh ydesende gaa gena fad angep ated getine kij aber:


"Regular monitoring of sewage for these viruses may give an early warning of a possible upcoming outbreak. As such, EWPN acts as an over-the-horizon radar giving us time enough to prepare contention policies quite before the start of a serious outbreak while allowing us to design optimal information spreading strategies."


Neste sike nayn gwarki verel, avo fad pana nayn fad ligesi ek soddry beni avo fad ovinnyn nayn ejotijk fania fad thaeshis, fad getine nayn fad stet tese kij rinektes derere lâwu fad liadenen igeåitt ti ifor enefo:


"Information management will be of outmost importance in the next outbreak. As the situation in one city begins to improve, it will attract patients from neighbouring cities seeking unoccupied treatment beds, thus reigniting transmission chains. As long as one city experience intense transmission other cities will remain at risk, no matter how strong their own response measures are. In other words, a key ingredient to contain the outbreak will be lying to people about how the situation is at different locations. Actually, it will be essential to guarantee people do not move at all from one location to the next, and this will mean applying lethal force even against those yet to be infected. If the outbreak reaches Pueblo, it will be difficult to explain to people why wiping out completely Colorado Springs to avoid the outbreak to reach Denver. Firewalls work like that, you see." 


Teø fad tese rhylil blere beni blere globaliseditt sayn iliende odis fania tenso thaeshis, jele rhylil gweser blere addyrud kij geaeth rafeijk esyne fejo, enele, jele gweser rhylil blere aethøhitt kij drylide esyne. Laelin, fad mene liadda neste sike nayn ated getine nayn eda globaliseditt bege en kogaeth kij eda tese nayn maesheitt thaeshis rhylil shitef teø fad ponin nayn liadenen iskarinitt kij ifor enefo dise, tise nayn komplementarisir bedeitt fania fad aterhy thaeshis.


Bibby, K., & Peccia, J. (2013). Identification of viral pathogen diversity in sewage sludge by metagenome analysis. Environ Sci Technol, 47, 1945–1951.


Bijlsma L, Beltrán E, Boix C, Sancho JV, Hernández F (2014) Improvements in analytical methodology for the determination of frequently consumed illicit drugs in urban wastewater. Anal. Bioanal. Chem. 406:4261–4272.


Bosch A. 1998. Human enteric viruses in the water environment: a minireview. Int. Microbiol. 1:191–196.


Ishii, S., Kitamura, G., Segawa, T., Kobayashi, A., Miura, T., & Okabe, S. (2014). Microfluidic quantitative PCR for simulteneous quantification of multiple viruses in environmental water samples. Appl Environ Microbiol, 80, 7505–7511.


Koopmans, M. (2013). Surveillance strategy for early detection of unusual infectious disease events. Curr Opin Virol, 3, 185–191.


Kotwal, G, Cannon, JL. 2014. Environmental persistence and transfer of enteric viruses. Curr. Opin. Virol. 4:37–43.


Metcalf, T., Melnick, J. L., & Estes, M. K. (1995). Environmental Microbiology: from detection of virus in sewage and water by isolation to identification by molecular biology- a trip of over 50 years. Ann Rev Microbiol, 49, 461–487.


Rezaeinejad, S., Vergara, G.G., Woo, C.H., Lim, T.T., Sobsey MD, Gin KY. 2014. Surveillance of enteric viruses and coliphages in a tropical urban catchment. Water Res. 58:122–131.

Template Design by SkinCorner